Senior Scientist I, Cell/Molecular Biology

• Independently conceive ideas and designs to investigate CHO expression technical improvements across both vector and host cell.
• Design and test dozens of vector constructs in recombinant stable CHO cell lines for increased mAb titer. Rapidly execute the associated transfections, screening, and clone-characterization studies to generate decision-quality data.
• Employ a variety of hands-on laboratory techniques, including transfection, aseptic CHO cell culture, DNA gene copy number analysis, RNA expression analysis (ddPCR / RT-qPCR), mAb titer measurement, cell engineering to knock in/out target genes.
• Operate and interpret data from core CLD instrumentation, including Octet (BLI titer), ViCell, CloneSelect Imager, microfluidic clone-screening platforms such as the Beacon, flow cytometry. Share responsibility for equipment maintenance.
• Apply a rigorous theoretical and practical understanding of recombinant protein expression from the genome to the protein - including genomic loci, epigenetics, transcription, mRNA stability, translation, the organelles and secretory machinery involved, and the engineering constraints specific to industrial CHO cell line development.
• Author and co-author cell line development technical reports and present data to the group and to leadership; contribute scientific insight to project teams.
• Collaborate closely with cell culture, analytical, purification, and bioinformatics colleagues to meet project timelines, and adhere to corporate standards for code of conduct, safety, materials handling, and good documentation practices.

Qualifications

• BS with a minimum of 10+ years, or MS with a minimum of 8+ years of experience, or PhD with 0-4 years' experience, in molecular biology, cell biology, biochemistry, or a related discipline.
• Demonstrated ability to read the literature, review external sources, and gather inputs to synthesize technically sound ideas and design proper experimental controls.
• Demonstrated ability to independently analyze experimental data, draw appropriate conclusions, and communicate them to a wide audience.
• Demonstrated proficiency with designing and implementing vector improvements to increase recombinant antibody expression levels in stable CHO cells.
• Proficiency with Geneious Prime or similar software for designing and annotating DNA vectors, primers, and probes, and performing sequence alignments.
• Proficiency with most or all of the following techniques and instrumentation: transfection, aseptic mammalian cell culture, Octet, ViCell, CloneSelect Imager, and microfluidic clone-screening platforms such as the Beacon.
• Demonstrated ability to learn, understand, and master new experimental techniques, and to multi-task and work within timelines.
• Strong scientific communication skills - verbal, written, and data presentation - with detailed daily digital lab notebook record-keeping proficiency.
• A candidate who combines hands-on wet-lab depth with the ability to think alongside the team and then execute independently.

• While we welcome all candidates with a genuine passion for CHO cell line development, we strongly prefer those who bring hands-on, industry cell line development experience in a CMC / drug-substance environment over candidates with purely academic or cell-culture-development-only backgrounds.
• Candidates with substantial experience developing CHO expression platform improvements in an industrial/CMC setting.
• Possess a demonstrated and genuine passion for getting the most mAb titer out of recombinant CHO cells.
• Previous experience performing cellular analyses using flow cytometry.
• Proficiency with cell engineering to knock in / knock out target genes using CRISPR/Cas or other suitable tools.

Additional Information

​Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law: ​

• The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location, and we may ultimately pay more or less than the posted range. This range may be modified in the future. ​
• We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees.​
• This job is eligible to participate in our short-term incentive programs. ​

Note: No amount of pay is considered to be wages or compensation until such amount is earned, vested, and determinable. The amount and availability of any bonus, commission, incentive, benefits, or any other form of compensation and benefits that are allocable to a particular employee remains in the Company's sole and absolute discretion unless and until paid and may be modified at the Company's sole and absolute discretion, consistent with applicable law. ​

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